HI Renee- I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried. It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered. We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat. We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining. The tissue lifting decreased dramatically- even on breast tissue. Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid "double-dipping" which can also cause tissue adherence problems.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
