We have the CBG recycler and use buffered formalin. Yes, you have to re-buffer it. They sell the buffer and also give you instructions on how to re-buffer the formalin.
Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Sunday, March 01, 2015 4:28 PM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: CBG recyler and recycled Formalin Bob Richmond makes an important point that should also be made clear by any company selling apparatus to recover formaldehyde by distillation of diluted formalin. The recovered product recovered from an aqueous fixative will be an aqueous solution of formaldehyde (and its low polymers), of uncertain concentration. Distillation of full-strength formalin (37%w/w = 40%w/v formaldehyde), done at atmospheric pressure, yields 20-30% of formaldehyde in the distillate, and leaves a higher concentration of formaldehyde and polymerization products in the still. Vacuum distillation or pressure distillation can change the yields to favour the collection of hydrated formaldehyde (methylene glycol) in the distillate. I'm summarizing Chapter 8 in Walker, JF (1964) Formaldehyde, 3rd edition. ISBN 0882752189. Walker's Chapter 8 tabulates conditions for distilling formaldehyde from various concentrations, and takes into account the methanol (about 5%, included in formalin to retard polymerization). The notion of an expensive machine for recycling formalin in a histology lab makes little sense. Why not simply filter (if necessary) and re-use the fixative after removing the specimen? It is still neutral, buffered formaldehyde. John Kiernan London, Canada = = = On 28/02/15, Bob Richmond <rsrichm...@gmail.com> wrote: > Ryan Roy HTL (ASCP) in Manchester NH asks: > > >>We are getting a new CBG that recycles xylene , alcohol, and > >>formalin. We > purchase buffered formalin. Does anyone know if after recycling the > recycled formalin would or would not need be re-buffered?<< > > If you distill buffered formalin, the formaldehyde is going to distill > over, but not the buffer phosphate, which will remain in the still > pot. I suppose you can buy phosphate mixtures to make Lillie's neutral > buffered formalin anew, using your recycled formaldehyde. > > I think you also have to measure the concentration of formalehyde in > the distillate, and dilute accordingly. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ===================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
