Dear Cecilia,
I hadn't seen the 2009 paper you mention. Please let me know the reference.
I've just sent you another email with an attached LM photo, which might be of
interest.
John Kiernan
= = =
On 25/03/15, "Cecilia A." <cecysta...@gmail.com> wrote:
> >
> > Hi John,
> >
> > Thank you for your reply.
> >
> > In a Developmental Dynamics paper in 2009, PTA was used as a "simple
> > staining method" that produced overall contrast for X-ray imaging soft
> > tissues similar to how Osmium Tetroxide was also used as a soak for this
> > purpose.
> >
> > We were interested in using PTA as it doesn't render the soft tissue black
> > like Osmium Tetroxide does, so that there's still a chance we would be able
> > to do further histology work on the tissue. However, we wonder what PTA
> > might do to the fixed yet unprocessed tissue when it is soaked for this
> > imaging purpose, and if it might affect its viability for staining and IHC
> > work.
> >
> > Thank you,
> > C.
> >
> >> On Wed, Mar 25, 2015 at 9:11 AM, John Kiernan <jkier...@uwo.ca> wrote:
> >> PTA can be used on tiny specimens to enhance electron-opacity. For light
> >> microscopy, PTA is used in the trichrome methods to enhance differential
> >> staining of collagen and cytoplasm, especially in paraffin sections. These
> >> uses of PTA are not the same as "soak formalin fixed soft tissue for a few
> >> hours before it gets processed". What are you hoping to achieve?
> >>
> >> John Kiernan
> >> London, Canada
> >> = = =
> >>
> >>> On 25/03/15, "Cecilia A." <cecysta...@gmail.com> wrote:
> >>> Hello Histonetters,
> >>>
> >>> I was wondering if anyone might be able to give some input regarding the
> >>> use of Phosphotungstic acid in which we want to soak formalin fixed soft
> >>> tissue for a few hours before it gets processed. Will subjecting it to PTA
> >>> before processing affect its viability for further histology work (ie, H&E
> >>> staining and IHC)?
> >>>
> >>> Thank you very much!
> >>> C.
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> >
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