I do a lot of Movat Pentachrome stains. Every once in a while something goes
wrong!
I just made a solution of Ferric Chloride as follows: Dissolve 12.4 grams
Ferric Chloride Hexahydrate in 500 ml dH2O. THen add 5.0 ml of concentrated
Hydrochloric acid.
What is troubling me is that the solution usually starts of brownish, then
turns clear yellow after I add the HCl. This time is stayed brownish and
didn't turn yellow.
Seems like 15 years ago we did a Movat where someone hadn't added the HCL to
the solution, it staed brown, and the Weigarts part of the stain didn't work.
Any ideas? I'm sure I added the HCl.
Jerry L Ricks
Research Scientist
University of Washington
Department of Pathology
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