Thank you for suggestions for softening beak.
Yes, I actually processed the whole head for decalcifying first and then place
the sample in 10% KOH for 30 mins. It was my first attempt to process beak
(including a whole head) thus, i did not know i should have processed softening
beak first.
Anyway, after placing the beak in 10% KOH for 30 mins, it did not seem to
soften enough. However, I did not overly place it in solution because it may
destroy the microscopic structure.
How soft the nail becomes after 10 % KOH or Nair treatment, such as you can
bent the nail easily etc..?
Any suggestion would be appreciated.
rui
***
From: ru...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Date: Fri, 10 Apr 2015 05:23:01 +0900
Subject: [Histonet] soften beak
Hi,
I am just wondering what you would recommend to soften avian beak for paraffin
processing.
I found a protocol that KOH could be used to soften keratin in nail, however, I
have not been able to find that this method is applicable specific to beak.
Another concern is if i use KOH for an entire head, does KOH affect (e.g.
damage) soft tissues?
Thank you in advance,
rui
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