Hi, Can anyone please let me know how to increase AEC sensitivity ?
I am using AEC for multiple IHC, but it is not particularly sensitive. My recepe is: 1% AEC in DMF 0.5ml 50mM acetate/sodium acetate buffer pH5.5 10ml 30% H2O2 5ul mix and filter incubate for 10-15min I m looking for enhancer (like imidazole for DAB) or more sensitive protocol for AEC. Thank you ​Hirata Masahiro Department of Diagnostic Pathology, Kyoto University Hospital hirat...@kuhp.kyoto-u.ac.jp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
