This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid). ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sue [suetp...@comcast.net] Sent: Friday, 24 April 2015 10:51 AM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact"
Hi All So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians. Sue Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
