In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen. This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation. Here is an excerpt from our procedure:
3. Label a 50 ml centrifuge tube with the cytology accession label. 4. Mix the specimen using a vortex mixer for 10-30 seconds. 5. Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube. 6. Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2). 7. Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size. Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen. I have document that illustrates this but I don't think the list serve will allow attachments. You can email me privately for this document. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
