Good article on IHC world about restoring nuclear detail to over decalcified tissue....it is called Problem Number 23. Good luck.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, April 29, 2015 12:21 PM To: pablo.sanc...@usc.es Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Over- decalcified tissue I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample? Sent from my iPhone > On Apr 29, 2015, at 9:08 AM, pablo.sanc...@usc.es wrote: > > As I usually process gross pieces of bone -that need abouth thirty hours > decalcifying- I suffer the same nuissance. Also would thank any hint. > > Pablo Sanchez > > > > Laurie Colbert <lcolb...@pathmdlabs.com> escribiu: > >> I have tissue that was left in decal over the weekend and now has very poor >> nuclear staining. Is there a "fix" for this so that I can get better >> nuclear staining (other than restaining for a long time in the hematoxylin)? >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged and >> confidential information, including patient information protected by federal >> and state privacy laws. It is intended only for the use of the person(s) >> named above. If you are not the intended recipient, you are hereby notified >> that any review, dissemination, distribution, or duplication of this >> communication is strictly prohibited. If you are not the intended recipient, >> please contact the sender by reply email and destroy all copies of the >> original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
