Amos, hello. Do you have a reference for this? All my files talk about "endogenous" B-gal in kidney and pancreas and other organs (but then article talks about after lacZ transgenic manipulation) or demonstration of alpha-galactosidase in kidneys or in senescence associated or lysozomal storage diseases or differentiating light background staining from lacZ with pH tricks or pictures of islet cells staining with surrounding exocrine pancreas staining or just the journalistic form of hand-waving "data not shown". Is beta-galactosidase readily expressed in completely normal kidney and where specifically? Thanks, Ray ----- Original Message -----
From: "Amos Brooks" <amosbro...@gmail.com> To: histonet@lists.utsouthwestern.edu Sent: Sunday, May 10, 2015 8:52:45 AM Subject: [Histonet] B-gal positive control Hi, Normal kidney should work fine for this. Amos On Fri, May 8, 2015 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Message: 9 > Date: Fri, 8 May 2015 14:50:42 +0000 > From: "Coffey, Anna (NIH/NCI) [C]" <anna.cof...@nih.gov> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] B-gal positive control > Message-ID: <5c3e10119a1b824fbe92b08279f74a9101799...@msgb10.nih.gov> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonet, > > Has anyone out there come across a good FFPE positive control for B-gal? > If so, please let me know! We would like to purchase a block or unstained > slides if at all possible. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
