Hi there,
thank you all very much for your comments and protocols on the
PAS-diastase. Although funny thing that cheaper option, I think I stick
to the Sigma enzyme first.
Update on the formalin "crystals". It is actually, as you suggested,
white granular precipitate. There was so much of it that it formed some
big "crusts" (which led me to think about crystals when looking trough
the bottle...). The formalin was aqueous solution (stabilised with 10%
methanol). I have tried to depolymerize it heating it with a couple of
NaOH2 beads but to no success. Thank you very much for your comments.
Regards
Julio
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet