Hi,
Sigma has discontinued selling type VIII-alfa amylase from barley
(catalogue ref A2771). Anyone knows any other commercial source of
this enzyme or if the other amylase (other types of alfa amylase or
beta amylase) work for PAS-D?
Thanks
Julio
Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> escribió:
Hi Julio,
Here is our procedure. It is very straight forward.
Beatrice
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-----Original Message-----
From: Julio Benavides [mailto:j.benavi...@eae.csic.es]
Sent: Friday, May 29, 2015 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS-diastase staining
Hi there,
I am trying to do a PAA-diastase in formalin-fixed embedded samples
(ovine liver). I would be most grateful if someone could give me a
hand in:
-Protocol (enzyme concentration, time of digestion...) for diastase
digestion (I am assuming that PAS afterwards is the normal protocol
for PAS) -A commercial source for diastase working in these samples.
Is the porcine amylase from sigma any good? cheaper options?
As always,
thank you very much for all your comments
Cheers
Julio
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