Dear Colleague I just want to remind you.. After all of that procedure of removing the paraffin sections from the positive charged slides. The slides will not be positive charged anymore and not suitable for IHC. Also the cost of the detergents and the chemicals which you used for removing the tissue section is more costly than the slides.
Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] Sent: Monday, June 22, 2015 5:53 PM To: 'Coffey, Anna (NIH/NCI) [C]'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Removing paraffin sections from glass slides This is what I would do... Soak the coverslip off in xylene Either - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry.. OR - air dry out of xylene and wipe off with a wet gauze - then do the alcohol dip Whichever worked best. Just my 2 cents.. Happy Monday!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.cof...@nih.gov] Sent: Monday, June 22, 2015 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing paraffin sections from glass slides Hello, I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet