Red bone and blue cartilage:

Webb, G.N. and Byrd, R.A. (1994). Simultaneous differential staining of 
cartilage and bone in rodent fetuses: an alcian blue and alizarin red S 
procedure without glacial acetic acid. Biotechnic & Histochemistry 69:181-185.

Redfern, B.G., Wise, L.D. and Spence, S. (2007). An alternative alcian blue dye 
variant for the evaluation of fetal cartilage. Birth Defects Research (Part B) 
80:171-176.

Yamazaki, Y., Yuguchi, M., Kubota, S. and Isokawa, K. (2011). Whole-mount bone 
and cartilage staining of chick embryos with minimal decalcification. 
Biotechnic & Histochemistry 86:351-358.

Trueman, D. and Stewart, J. (2014). An automated technique for double staining 
mouse fetal and neonatal skeletal specimens to differentiate bone and 
cartilage. Biotechnic & Histochemistry 89:315-319.

John Kiernan
London, Canada
P.S. Fetus is a 4th declension Latin noun, so its plural in English is fetuses, 
not feti! 
= = =
On 06/07/15, "Wilson, Carol"  <carol.wil...@ricerca.com> wrote:
> Good Day All,
> Can someone pass along methodology for processing rodent Feti for Alizarin 
> Red stain and exam? I would appreciate any input/ direction given as this is 
> a foreign topic to me.
> Thanks,
> Carol
> 
> Carol Wilson, HT(ASCP)
> Associate Scientist III
> Team Leader/Histopathology
> Ricerca Biosciences, LLC
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> Histonet@lists.utsouthwestern.edu
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> 
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