Happy Friday1 We are experiencing the same problem. Just had a site visit from Ventana and they suggested using an antibody dilution buffer. Use an option kit filled with Ventana Product ADB250 (antibody dilution buffer). Check options in protocol editing and 8 minutes, suggested time. Have not tried it but inted to. Good luck.
-----Original Message----- From: Susan Foreman [mailto:sfore...@labpath.com] Sent: Friday, July 10, 2015 5:08 AM To: 'Sebree Linda A'; 'Cartun, Richard'; 'Histology Technician'; 'Histonet'; histonet-requ...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra using UltraView DAB detection. He have had dirty staining for spirochetes (syphilis) in the past utilizing the Red Detection kit and decons do seem to help a bit. The DAB detection kit is much cleaner. -----Original Message----- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, July 09, 2015 5:16 PM To: 'Cartun, Richard'; Histology Technician; Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Thursday, July 09, 2015 4:06 PM To: Histology Technician; Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Histology Technician [mailto:histology81...@att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
