We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also.
Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax mwh...@mhs.net -----Original Message----- From: Jeffrey Robinson [mailto:jrobin...@pathology-associates.com] Sent: Thursday, July 30, 2015 1:06 PM To: Cates, Julia; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana H.Pylori antibody Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
