Wow! Thank you all so much for your responses. They have been very helpful;
it's so great to have a community of professionals that are so willing to give
of their knowledge.
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
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Message: 1
Date: Thu, 30 Jul 2015 17:06:04 +0000
From: Jeffrey Robinson <jrobin...@pathology-associates.com>
To: "Cates, Julia" <julia.ca...@ahss.org>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:
<204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local>
Content-Type: text/plain; charset="us-ascii"
Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I
don't recall using the Ventana H. pylori but I was using the CellMarque H.
pylori for years. It would often have a lot of background as you describe. I
run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run
on the Benchmarks were usually cleaner than those run on the Bond but still not
as clean as I would like. I finally switched over to BioCare's H. pylori after
many complaints from my pathologists. It has been much cleaner on all of my
IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have
ever seen. The slides run on the Bond may still have a little background but
it is still much cleaner than the ones I used to run with the CellMarque
antibody. One of my pathologists who used to complain about the excessive
background now calls it his "go to" stain and is very happy with how crisp it
is. The organisms stand out very nicely. BioCare will send you a free sample
if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-----Original Message-----
From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody
Good day Histonet,
I have seen in the past conversations regarding Ventana's H.Pylori antibody and
how dirty looking the stain can be. We have been using this antibody for
several years now and have never really been happy with the quality. We have
tried the recommended methods to "clean up" the stain and still we run into
repeating the stain and/or complaints from the pathologists. We are
considering using a different vendor but my concern is that my efforts will be
a lateral move. Is anyone using a product that produces a clean stain or is
this something inherent to this antibody? Are the pathologists happy with it
and not just tolerating it?
Thank you for your suggestions,
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
Confidentiality Statement: This email message, including any attachments, is
for the sole use of the intended recipient and may contain confidential and
privileged information. Any unauthorized review, use, disclosure, or
distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply to this email and delete the original and all
copies of this email.
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