Hi all, A few weeks back I posted a question about identification of lymphatics in the axolotl (see below). John Kiernan suggested that enzyme histochem on paraffin sections might be possible, as per an article by Okada et al in 1994. I have been trying to hunt down this article but so far to no avail. Does anyone have a copy they may be able to scan and send me? It is in a procedings supplement, Lymphology journal:
Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. Book title is: Progress in Lymphology XIV—Proceedings of the XIVth Congress in Washington DC, 1993, MH Witte, CL Witte (Eds.): Lymphology 27(Suppl): 1-893, 1994. Many thanks , Jason -- Jason Palmer Histology Laboratory Coordinator O'Brien Institute / St Vincent's Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpal...@svi.edu.au ----- Original Message ----- From: "John Kiernan" <jkier...@uwo.ca> To: "Jason Palmer" <jpal...@svi.edu.au>, histonet@lists.utsouthwestern.edu Sent: Wednesday, 29 July, 2015 2:06:29 AM Subject: Re: [Histonet] axolotl lymphatics Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references. Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24 :125-129. Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27 , Suppl:106-107. Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51 :331-338. Needless to say, none of these relate to amphibian tissues! John Kiernan Anatomy, UWO, London, Canada = = = On 26/07/15, Jason Palmer via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hi all, I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? Thanks, Jason _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
