________________________________________
From: Histology [hi...@pathlab.us]
Sent: Monday, October 05, 2015 5:18 AM
To: Morken, Timothy
Subject: RE: GMS and Gram stains

1.  Gram:  Sigma-Aldrich Accustain
2.  Gram:  Hard to see gram negative

1. GMS:  Grocott's Methenmmine Silver modified
2. GMS:  Switched from microwave to oven.  Too long (2 hours) and can't seem to 
get the fungi dark enough.

Thanks

-----Original Message-----
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Friday, October 02, 2015 12:16 PM
To: Histology
Subject: RE: GMS and Gram stains

Mehndi,
These procedures are so common that without more information you are likely to 
get a lot of people sending you procedures that are identical to what you now 
use.

The questions we will ask are:
1) what procedures are using now?
 and
2) what is it about your current procedures  that is not acceptable?



Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Histology via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, October 02, 2015 4:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GMS and Gram stains

Hi All Netters,

I need to get a better GMS and Gram stain.  If anyone could share their 
protocols where they get the solutions that would be fantastic.

Thanks,

Mehndi Helgren

Dominion Pathology Labs
Norfolk, VA (AKA Hurricane Central)  :)


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