We had this happen when the individual changing the processor reversed order of the alcohols, placing the newest first and the oldest last. Hope your answer is as simple, even if it is embarrassing. Joe Saby
From: "Abbott, Tanya via Histonet" <histonet@lists.utsouthwestern.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, October 14, 2015 4:35 PM Subject: [Histonet] VIP5 Help!!! We changed our processor on Monday, all reagents. Run Monday into Tuesday a.m. was fine. Tuesday nights run had some blocks in it that weren't fully processed; chamber looked like it had droplets of residual water (or maybe alcohol?) in it. Dumped and refilled everything, ran a few test blocks, same scenario. Any suggestions?! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet