We do this routinely at the Shriners Burns Hospital in Galveston when asked to look for fungi in infected burn wounds and provide a "rapid section" report. It works very well when the timing is carefully controlled by frequent visual inspection. We use a paraffin section as a control. We also run a tissue Gram stain on these samples and report the results in about two hours.
________________________________________ From: Pedro Louro via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, November 11, 2015 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS silver stain on frozen tissue Hi everyone, Has anyone experimented with performing a Grocott Methenamine Silver stain on frozen tissues? I have a PI that is requesting this but I cannot find any literature on frozen tissue. Any help or ideas is appreciated. Thanks in advance, Pedro _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
