Skin is often difficult to cut. Not sure I have seen that artifact, but here is what I use when having difficulties. Gently blot the tissue/epidermis dry before immersing in liquid OCT/Neg50. Then freeze in cryostat. Avoid refrigerant spray. Sometimes we allow tissue to sit in oCT for 5 min and then freeze. If tissue was in 20% sucrose, we blot, then rinse in OCT for 5 min then replace/mount in new OCT and freeze.
Steve A. McClain, MD > On Dec 6, 2015, at 13:10, "histonet-requ...@lists.utsouthwestern.edu" > <histonet-requ...@lists.utsouthwestern.edu> wrote: > > [Histonet] Oct separating from epidermis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
