Do you using "freezing spray" on these specimens? Direct spray can cause the tissue to appear burnt.
From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> To: histonet@lists.utsouthwestern.edu Date: 01/05/2016 06:00 AM Subject: [Histonet] Processing of breast tissue We have been experiencing some issue lately with cutting our breast excisions. Recently we had two specimen where after our 8 hr run the tissue was still extremely soft. Our medical director said the fat sectioned beautifully but the fibrous tissue appeared cooked. Can anyone give a suggestion as to how we should process our breast excisions from grossing through to microtomy sectioning. Currently I have tried to get grossing to limit the specimen to 2mm by 2mm by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior processor. We cut our sections at 4um but have recently had to do them at 6um in order to get the soft samples cut. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet