James We use 10% Formic Acid to decal mouse femur and tibia - The rear legs are removed at the hip with a pair of rongeurs the skin is removed to the ankle, we allow the knee joints to fix for 48 hours, they need to be in a normal degree of flexion, meaning you want them in a container that does not bend the knee. We trim off the muscle and place in decal for overnight - process the next day on an hour processing cycle. You don't need microwave for mouse knees they decal quickly. Most of the work we do is for cartilage degeneration, rheumatoid or osteoarthritis, if you are looking at bone marrow I would modify slightly. I can send a work instruction document that has more information in another e-mail if you are interested.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Herrick, James L. (Jim), MSA via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, January 11, 2016 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse femur decal and paraffin embedding protocol Dear Histonet Colleagues, I hope the holiday season has been good to all. We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time. It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!! Jim Herrick, M.S.A. Supervisor - Sr. Res.Tech. Department of Orthopedics Biomaterials and Histomorphometry Core Lab Biomaterials and Tissue Engineering Lab Office Phone: (507) 538-4300 Lab Phone: (507) 255-5946 Pager: 127 (13239) Fax: (507) 266-9451 Email: herrick.ja...@mayo.edu<mailto:herrick.ja...@mayo.edu> ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org<http://www.mayoclinic.org/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
