Hello, I am having a problem with sectioning.
I'm cutting 5 um sections but with the turn of the microtome I will miss the block, or take a small piece of the block/tissue and then the next section ends up thick. This missing can take several turns of the wheel so I can get really big sections. I have checked the angle of the knife to the block and the tension along the blade but these are fine. I worry that the issue might be temperature. The microtome is on a bench against an outside wall. The temperature is about 15 oC. Could this be the issue? I am not cooling the block on ice. Previous experience with block-cooling was that I would get thick and thin sections that way... this is a very different problem. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet