We use the bromocresol and it does stay yellow if the pH is correct. We also use a narrow range pH strip. You might want to check the pH of your DI water.
Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Mon, Feb 1, 2016 at 1:54 PM, Samantha Sackmann via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I have tried this procedure multiple times and everytime the solution > turns purple in the glassware filled with deionized water, even after > rinsing in running water for 2+ minutes. We do not have a dishwasher, so > all the glassware is hand-washed. Could there be a problem with our DI > water? Has anyone else had an issue with this? > > Thanks, > Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II > CellNetix Pathology & Laboratories > 800 West 5th Avenue > Spokane, WA 99204 > 509-473-2311 > sedwa...@cellnetix.com > > > > > > > > > > What is everyone using to test their glassware for detergent residue? Is > there a ready to use solution or not? > > > > > > CAP Checklist... > > > > Glassware Cleaning Phase II > > > > There are appropriate documented procedures for handling and cleaning > glassware, including > > methods for testing for detergent removal. > > > > NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. > must be included. > > > > A simple procedure to check for detergent residue uses bromcresol purple > (0.1 g bromcresol purple > > in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled > water into a representative, > > washed, glassware item. Add two to three drops bromcresol solution. A > purple color reveals residual > > detergent. A yellow color indicates satisfactory rinsing. > > > > Thanks! > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark <@t> sfmc.net< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > http://www.sfmc.net<http://www.sfmc.net/> > > > > > Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II > CellNetix Pathology & Laboratories > 800 West 5th Avenue > Spokane, WA 99204 > 509-473-2311 > sedwa...@cellnetix.com > > DISCLAIMER: > > This message is intended for the sole use of the addressee, and may > contain information that is privileged, confidential and exempt from > disclosure under applicable law. If you are not the addressee you are > hereby notified that you may not use, copy, disclose, or distribute to > anyone the message or any information contained in the message. If you have > received this message in error, please immediately advise the sender by > reply email and delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet