Thanks for these excellent comments on troubleshooting the EA and OG-6 components I've copied them for my files. The important thing is to have frequent feedback from the cytotechnologist to whoever is doing the staining.
In one job I had the staining was done by a clerical person who took great pride in the fact that she'd never looked down a microscope in her life. (She never took a day's vacation - always an eosinophlic clupeid, as any accountant will tell you - got caught with her hand in the till - I hope her jump suit's dyed with orange G.) The cytotechnologists were moonlighters in a distant place - we saw them once a year at the Christmas party. I got burned doing the 10% QC - we had no way to feed back if we found an error - we all missed a cervical adenocarcinoma - only time I've been sued in >50 years in practice. In another crazy practice the cytotechnologist only worked at night, and was forbidden - for reasons I never understood - to ever be in the hospital in the daytime - she'd never even met the histotechnologist who did her staining, and who never looked at a slide. Fortunately we didn't get sued, but we certainly could have. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet