For the record please note that I have over thirty-six years experience working in a Histology lab. I have been a supervisor or manager of a hospital and clinic histology department for at least 25 years.
Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> From: Jamal Rowaihi [mailto:j.rowa...@alborglaboratories.com] Sent: Tuesday, February 16, 2016 1:56 PM To: Manfre, Philip; Rene J Buesa; Vickroy, James Cc: جمال الرويحي; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Great, I agree Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories Sent from my cell phone -------- Original message -------- From: "Manfre, Philip via Histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa <rjbu...@yahoo.com<mailto:rjbu...@yahoo.com>>, "Vickroy, James" <jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>> Cc: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain → "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.rené On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com%3cmailto:jvick...@springfieldclinic.com>> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. 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