Hi,
in my experience, Bouin´s fixative can sometimes give you problems
with IHC. Both because it seriously interfere with some antigens (in
my case, I was doing IHC for lentivirus and herpesvirus and more than
24h of fixation would drastically reduce IHC labelling) but also
because it gives you non-specific labelling (in may case, ovine
neutrophils came up positive with that lentivurs Ac).
Julio
Robert Byrd via Histonet <histonet@lists.utsouthwestern.edu> escribió:
Bouin's solution is an excellent fixative for fetal brains, we were still
using it routinely when I trained 10 years ago but has (and already had in
most places) largely fallen out of use due to the explosive risk associated
with dried picric acid residue in drainpipes etc. It does work remarkably
well on soft and macerated fetal brains though. I use 10% NBF now out of
necessity but might be able to track down a Bouin's protocol if you are
interested.
On Tue, Feb 23, 2016 at 5:11 PM, Hawkins, Hal K. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
We briefly experimented with using a fixative for stillborn fetal brains
that was a mixture of equal volumes of 20% formalin and 95% ethanol. The
gross preservation and firmness of these very soft brains was improved, and
the histology results were fine, but as one might predict, some antigens
were not preserved for immunohistochemistry.
________________________________________
From: Jeff Jurczak via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 23, 2016 3:45 PM
To: Bob Richmond
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Davidson's fixative and IHC question
Is this a good fixative for fetal brain? Anybody have any suggestions as
to protocols? Do any of the readers here do fetal (human) brain on a
regular basis?
----- Original Message -----
From: "Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu>
To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu
>
Sent: Tuesday, February 23, 2016 3:22:50 PM
Subject: Re: [Histonet] Davidson's fixative and IHC question
Several queries about the possible effect of Davidson's fixative on
immunohistochemistry.
I have a lot of experience with Davidson's fixative, but none with its
effect on IHC.
Davidson's fixative is easily prepared:
3 parts tap water
3 parts ethanol or reagent alcohol
2 parts 37% formalin (not neutral buffered formalin)
1 part glacial acetic acid
It is quite destructive to H & E staining if fixation is at all prolonged.
Tissue should be taken out of it in not more much more than 24 hours.
The history of Davidson's fixative is somewhat obscure - I have a good bit
of information on it. John Kiernan has said he'll work on ascertaining more
about its history if he ever has the time.
Bob Richmond
Samurai Pathologist
Maryville TN
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