Hi,

I have a question regarding the use of UCSF Collodion Bag Cell blocks. once you do the bag, you pour in the cells in, let´s say 10ml of formalin. Then, how do you proceed? Do you spin down the cells with the bag inside a plastic tube? do you take then put the formalin and cut the top of the bag into a cassette? do you close the bag with a thread with the cells and formalin?....

Any help with this would be greatly appreciated,

Thanks a lot

julio



WILLIAM DESALVO via Histonet <histonet@lists.utsouthwestern.edu> escribió:

What method are you using? Making your own coated tubes, UCSF or MD Anderson. Are you purchasing coated tubes? Once the tube is coated, dried (using hood) and filled w dH2O, there should be no odor. Always work w/ collodion under the hood. Ethyl ether is main component. Once in cassette and processed, there should not be odor.

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________________________________
From: Baldwin, Kathy via Histonet<mailto:histonet@lists.utsouthwestern.edu>
Sent: ‎1/‎4/‎2016 2:07 PM
To: 'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Collodian bag for cell blocks

Hi All
Was wondering if anyone is using the Collodian bag for cell blocks. We just got it and have been using it 'works great' but all the techs are complaining of the smell.. Our ventilation has been looked at and has passed however the smell still lingers in the room. Any suggestions??


Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232
Cell 812-887-3357





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