"Another theory floating in the lab is because we bleach the stain buckets over the weekend maybe the bleach is saturating the bucket and slowly leaching out diluting the hematoxylin later in the day. (I would think this would be the case for all the slides not just the last few)"
I guess I need to give more details...the first few runs look "fine" per my supervisor. If we didn't rinse the buckets I would think the first few runs would look pale too...Leaching out bleach later in the day seems illogical to me. This is why I am leaning toward the xylene being saturated (but we didn't have half the cases that we normally do and it was fresh Monday morning when this occurred) Or the tap water which is hooked directly to our stainer and can vary from clean to yellow to tan within a day. I had this issue with the tap water when I was at another facility a few block from this one in the same city. I just need another voice of reason to show my supervisor. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: cda...@che-east.org www.saintfrancishealthcare.org ________________________________________ From: Podawiltz, Thomas [tpodawi...@lrgh.org] Sent: Tuesday, February 23, 2016 1:28 PM To: Cassie P. Davis Subject: RE: H & E Troubleshooting Hi Cassandra, Does the Eosin looked washed out. If so it may be your bluing. I had this issue a year or so ago and it turned out that the bluing was to strong and it was effecting the ph of the Eosin and giving it a washed out look. Since I had a gallon of the stuff, I just adjusted the time in bluing and my water rinse and the issue went away. I will say I have also had the issue with bleached containers, but when that happened everything look light. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 23, 2016 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H & E Troubleshooting Hi Histo folks, We are having a fun time troubleshooting our H&E because the problem in not consistent... Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcohol since our ordered had not arrived yet. One of our doctor said our hematoxylin & eosin was readable but too light(he got 6 out of the 36 cases), the other (who got the 30 cases)said it was good. We had half the number of slides going through staining than we normally do. We don't let the last alcohol before xylene get pink from eosin. Had it been a Thursday-Friday I would be concerned the xylene needed to be changed. The Define and Bluing were made fresh. My theory is the tap water could be off or the xylene needs to be changed. Another theory floating in the lab is because we bleach the stain buckets over the weekend maybe the bleach is saturating the bucket and slowly leaching out diluting the hematoxylin later in the day. (I would think this would be the case for all the slides not just the last few) Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: cda...@che-east.org<mailto:n...@che-east.org> www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
