In this space there is the cryojane (expensive, http://www.leicabiosystems.com/research/neuroscience/details/product/tape-transfer-system/) or the Japanese Kawamoto method: http://section-lab.jp/English/Introduction.htm
I have also found that if you take a regular piece of scotch tape and put it on the block prior to sectioning that it also works :) (trim to your area of interest, move the blade along, place on the tape and press down (use a tool to do this - don't use your hand to reduce the warming of the tissue, which would give too big a slice), then flip the scotch tape to the outside of the blade and take a section. You can then stain the tissue on the tape, dehydrate and clear, then stick the whole thing under a coverslip. Crude but effective for standard stains. I could not get immunostaining to work with it though...... It is hard to then get the tissue off the tape - apparently it works with hexane for the Kawamoto method, but I could never get that to work Ultimately if you were going to buy something I would say the Kawamoto is a great place to start, they (he) has a bunch of good tools in cute boxes that you can work with to make the process easier Good Luck! mills On Wed, Mar 2, 2016 at 11:36 AM, M.O. via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > I am looking into options for cryosectioning undecalcified mouse knees. > One of my co-workers said he used a film in Japan that stuck to the > cryoblock and then would do IHC directly on this tape. > > Has anyone cut undecalcified cryosections? If so, what options are there > and do you recommend one over the other? > > Thank you, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet