Hello Histonetters, I was wondering if anyone could offer advice for the processing of our human brain xenografts harvested from mice. The tumor specimens are approximately 0.5 x 0.5 x 0.5cm. We have been putting them on our animal program ( 30 min alcohols) but once embedded, the tissue looks brittle and some shrinkage occurs. The morphology looks okay. S hould we put them on our routine program ( 1 hour ethanols and xylenes) or is brain tissue too delicate for that run? Would appreciate any advice.
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