I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone 
specimens with metastatic breast cancer.  I compared the results with other 
specimens from the same patient (usually the primary breast cancer) that were 
not decalcified.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 05, 2016 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Validating IHC on decalcified specimens

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers 
on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com<mailto:tejohn...@genoptix.com>
www.genoptix.com<http://www.genoptix.com/>


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