We have had troublemakers and rabble rousers on HistoNet before who just are not happy without causing everyone discomfort by their manners/words. The more we feed this and complaint about the worse it will get for all of us. We don't need to answer or get upset just delete and movew on. This is the same as the school yard bully who needs attention anyway they can get it and now they can use the internet and words instead of fists and threats. In this case it is a keyboard and something to make us mad. DELETE and consider the source as my Grandmother would say, "Don't get in the gutter with them". Histology has a great tool and interface for us here so let us focus on the positive more.
Pam Marcum -----Original Message----- From: Hudson, Pamela R ASHVAMC via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2 OMG This is terrible. I can't believe I belong to a group of such unprofessional people. Pamela R. Hudson, HT (ASCP) Charles George VA Medical Center Pathology Department 1100 Tunnel Road Asheville, NC 28805 828-298-7911 x5747 pamela.hud...@va.gov -----Original Message----- From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2 Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. ________________________________________ From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Tuesday, May 3, 2016 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD <lr...@uropartners.com> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer <cjbulmer2...@aol.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> To: Lester Raff MD <lr...@uropartners.com>, "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet@lists.utsouthwestern.edu> wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf <lindamarg...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31cf79c7-84c3-45b8-af7b-20151c22f...@gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer <cjbulmer2...@aol.com> > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-ow...@lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" <philip_man...@merck.com> To: Rene J Buesa <rjbu...@yahoo.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558a4571351d0c42bd923f403f4198c40111d88d1...@usctmxp51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet@lists.utsouthwestern.edu> wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: Cindy Bulmer <cjbulmer2...@aol.com> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] IHC positive controls Message-ID: <761e2b5697f795489c8710bcc72141ff6fd47...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" <dkb...@chs.net> To: "Manfre, Philip" <philip_man...@merck.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7eafe982e328304da6ce2b677bb76246ab4b7...@tn001wexmbx014.us.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet@lists.utsouthwestern.edu> wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO <wdesalvo....@outlook.com> To: "Manfre, Philip" <philip_man...@merck.com>, Rene J Buesa <rjbu...@yahoo.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <col402-eas17105428354e8afe011831082...@phx.gbl> Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa<mailto:rjbu...@yahoo.com> Cc: 'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern .edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet@lists.utsouthwestern.edu> wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller <mi...@3scan.com> To: Lester Raff MD <lr...@uropartners.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <CAFTHRQNbrcnN4jyGQypUYEFPrPdyPVjSjJQC=2w4+orwtth...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet@lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is > for the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added > to my mailing list for future notifications, just drop me a line at > les.r...@post.com<mailto:les.r...@post.com> The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to > let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist > discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO <wdesalvo....@outlook.com> To: STEVEN PINHEIRO <spinhe...@lumc.edu> Cc: <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <col129-w27de73aed699c88244aa9582...@phx.gbl> Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: spinhe...@lumc.edu > To: wdesalvo....@outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet > [mailto:histonet@lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via > Histonet<mailto:histonet@lists.utsouthwestern.edu> > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa<mailto:rjbu...@yahoo.com> > Cc: > 'histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthweste > rn.edu> > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet > [mailto:histonet@lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet@lists.utsouthwestern.edu> wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.r...@post.com<mailto:les.r...@post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, > Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct > contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa <rjbu...@yahoo.com> To: Linda Margraf <lindamarg...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.javamail.ya...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet <histonet@lists.utsouthwestern.edu> wrote: > From: Cindy Bulmer <cjbulmer2...@aol.com> > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-ow...@lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? >and put slides ? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone <ksime...@leavittmgt.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] HHV-8 RTU Message-ID: <43944b1dbaac2846b7b9d626b5f1233cc9185...@vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" <pamar...@uams.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207...@mail13m2n1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
