As far as I remember the incubation temperature is at roomtemperature. 60°C would rather denature the native enzyme than increase activity.
Look at the optimal working temp of the reagens you have bought. regards Gudrun -----Ursprüngliche Nachricht----- Von: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Mittwoch, 04. Mai 2016 22:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet