Can someone provide the link for the application to obtain HT Florida State Licensure
Sent from my iPhone > On May 10, 2016, at 12:00 PM, <histonet-requ...@lists.utsouthwestern.edu> > <histonet-requ...@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. IHC Wet Workshop announcement (Ihc Workshop) > 2. Re: PAS Stain (Seeley, Heather) > 3. Re: Tracking surgical specimens (Jamieson Anderson) > 4. Tips on staining PLA2R assay on Leica Bond (Eddie Martin) > 5. Re: PAS Stain (victor_tob...@comcast.net) > 6. P16 (Charles Riley) > 7. Cytology/Histology Staining Question (Mullen, Mary) > 8. Re: Cytology/Histology Staining Question (Rene J Buesa) > 9. ASCP Certified Pathologist Assistant Job Opening- (Melissa Owens) > 10. Melanin Bleach (Heckford, Karen - SMMC-SF) > 11. Re: Melanin Bleach (Jeffrey Robinson) > 12. Re: Melanin Bleach (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 9 May 2016 10:16:39 -0700 > From: Ihc Workshop <ihcworks...@gmail.com> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] IHC Wet Workshop announcement > Message-ID: <3fac1e78-7c1b-4cbc-9a99-fda3f2baf...@gmail.com> > Content-Type: text/plain; charset=us-ascii > > June 23 & 24, 2016. San Francisco Bay Area > This IHC lab course is aimed at hands-on training of attendees in all > aspects of IHC staining of human, mouse and animal tissues with varied > antibodies. This is a small group workshop and is taught in a lab.....Lots of > troubleshooting, only few spots left. > To get more detail contact. Maria at: ihcworks...@gmail.com > > > > > ------------------------------ > > Message: 2 > Date: Mon, 9 May 2016 17:35:02 +0000 > From: "Seeley, Heather" <heather.see...@tenethealth.com> > To: Bob Richmond <rsrichm...@gmail.com>, > "Histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] PAS Stain > Message-ID: > <9dfe334e776e734d9d231ef60cce93c57e26d...@tenhdcthmb10-31.tenethealth.net> > > Content-Type: text/plain; charset="us-ascii" > > We have one girl that always spits on our slide! :) works great! > > HEATHER SEELEY, HT(ASCP) > Histotech > 803-985-4676 OFFICE > 803-327-7598 FAX > > > ________________________________________ > From: Bob Richmond [rsrichm...@gmail.com] > Sent: Thursday, May 05, 2016 2:10 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Amylase (diastase) for the PAS stain queries: > > Whatever happened to spitting on the slide (30 min at room temperature)? > John Kiernan advises "thinking of lemons and drooling into a small beaker" > though I'd advise chewing on a rubber band for a few seconds. > > He notes that alpha amylase is preferred. I'd go with the cheapest one in > the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma > offers a heat-stable alpha amylase. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Mon, 9 May 2016 17:41:09 +0000 (UTC) > From: Jamieson Anderson <jamiesonander...@gmail.com> > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Tracking surgical specimens > Message-ID: > <537327fdd6ba9f6c.0b786cd3-7fd7-4574-b1dc-3d754eacb...@mail.outlook.com> > > Content-Type: text/plain; charset=us-ascii > > Hi Lynne, > In our lab we have a log book at Accessioning that each > physician/nurse/porter/MOA signs when dropping off specimens. We also > encourage them to drop off a QC sheet with a patient label for each specimen > that is dropped off. We check these to ensure we have received each specimen, > and we date/time stamp them and keep them on record in case we need to refer > back to them in the future (ie. In case a clinician claims they dropped off a > specimen we can prove they did not). > Jamieson AndersonTechnical Coordinator - Anatomic PathologySt. Paul's > HospitalLower Mainland Pathology & Laboratory Medicine > > _____________________________ > > Date: Mon, 9 May 2016 14:49:56 +0000 > From: "Bell, Lynne" <lynne.b...@cvmc.org> > To: "Histonet (histonet@lists.utsouthwestern.edu)" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Tracking surgical specimens brought to the lab > Message-ID: > <ef83d5d097374d4497af4622c35be29301862aa...@cvmc-emailmbx1.cvmc.org> > Content-Type: text/plain; charset="us-ascii" > > For those of you who have specimens brought to your lab: do you have the > person dropping off the specimen(s) initial a manifest to keep track of > specimens actually dropped off. Case in point - a physician's office says > they dropped off a specimen and we have no record of it being accessioned. I > would like to track specimens dropped off and I am not sure how to accomplish > this. > > I appreciate any feedback! > > Thanks, > > > Lynne Bell, HT (ASCP) > Histology Team Leader > Central Vermont Medical Center > 130 Fisher Road > Berlin, VT 05641 > (802)371-4923 > > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 150, Issue 12 > ***************************************** > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 9 May 2016 12:51:46 -0500 > From: Eddie Martin <edmarti...@gmail.com> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Tips on staining PLA2R assay on Leica Bond > Message-ID: <16edc8ec-edab-4f63-a3bd-bec24eab0...@gmail.com> > Content-Type: text/plain; charset=utf-8 > > I don?t do staining for this antibody, but have experience with IMF on frozen > tissue and FFPE and have worked with the Leica BOND max and BOND-3. If you > would prefer IHC over IMF, you can search for a human anti-mouse, or human > anti-rabbit antibody. Abcam provides a polyclonal whole serum antibody that > works on FFPE. I grabbed this from their website: > Anti-PLA2R antibody (ab80054) > > Since Abcam?s PLA2R antibody is a rabbit anti-human, you would need to create > a modified DAB or modified RED protocol that doesn?t include the secondary > antibody / linker step in your protocol prior to dispensing polymer. The > rest of your protocol can remain as it is. > > As you are using a Leica BOND, both ways are actually pretty easy to set up, > either indirect IMF or using Leica?s DAB kit for IHC. You would just need to > play around with retrieval times with both Citrate and EDTA to get the > optimum staining pattern. Additional ancillaries may be added when necessary > should you have any background stain that is hard to get out. > > Best Regards, > > Eddie Martin, HT(ASCP), QIHC > 954-826-9403 > edmarti...@gmail.com > > ------------------------------ > > Message: 5 > Date: Mon, 9 May 2016 22:39:38 +0000 (UTC) > From: victor_tob...@comcast.net > To: Heather Seeley <heather.see...@tenethealth.com>, > "Histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu>, Bob Richmond > <rsrichm...@gmail.com> > Subject: Re: [Histonet] PAS Stain > Message-ID: > <1237907839.22424188.1462833578398.javamail.zim...@comcast.net> > Content-Type: text/plain; charset="utf-8" > > > > ------------------------------ > > Message: 6 > Date: Tue, 10 May 2016 05:47:18 -0400 > From: Charles Riley <cri...@dpspa.com> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] P16 > Message-ID: > <caaqhb13e__iaj-x1fgn7zkre1_bmkrrgfi2zftsgkrzrrav...@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Has anyone found a way to do p16 staining without purchasing anything from > Ventana? > > My company wants to do P16 but refuses to by any ventana products and I > have explained it is a waste of money to keep testing the antibodies from > all the other companies. > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > > ------------------------------ > > Message: 7 > Date: Tue, 10 May 2016 14:54:55 +0000 > From: "Mullen, Mary" <mulle...@mail.magee.edu> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Cytology/Histology Staining Question > Message-ID: > <374dc72e6b29d44086f8ff3289351b2508823...@msxmbxnsprd39.acct.upmchs.net> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello all, > > > > I work in a small, low volume community hospital and was recently asked by a > coworker why we do not just run both our cytology and histology slides on the > same automated stainer (with their respective protocols). > > > > What I am wanting to know is if there is anyone currently running both > staining protocols on a single automated stainer using common > alcohols/xylenes/water? What are the pros/cons? Has there been any > cross-contamination issues? > > > > We only run non-gyn cytology, all gyn cytology is sent out. > > > > > > > > Thanks, > > > > Mary K. Mullen, HTL(ASCP)CM > Histotechnologist > UPMC Northwest > Seneca, PA > > > > ------------------------------ > > Message: 8 > Date: Tue, 10 May 2016 15:07:04 +0000 (UTC) > From: Rene J Buesa <rjbu...@yahoo.com> > To: "Mullen, Mary" <mulle...@mail.magee.edu>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Cytology/Histology Staining Question > Message-ID: > <1252522947.1519943.1462892824143.javamail.ya...@mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > I would be concerned with potential cross-contamination. In my lab we had 2 > staining instruments, one for cytology and other for histology.Ren? > > On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" > <histonet@lists.utsouthwestern.edu> wrote: > > > Hello all, > > > > I work in a small, low volume community hospital and was recently asked by a > coworker why we do not just run both our cytology and histology slides on the > same automated stainer (with their respective protocols). > > > > What I am wanting to know is if there is anyone currently running both > staining protocols on a single automated stainer using common > alcohols/xylenes/water? What are the pros/cons? Has there been any > cross-contamination issues? > > > > We only run non-gyn cytology, all gyn cytology is sent out. > > > > > > > > Thanks, > > > > Mary K. Mullen, HTL(ASCP)CM > Histotechnologist > UPMC Northwest > Seneca, PA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Tue, 10 May 2016 15:44:21 +0000 > From: Melissa Owens <meli...@alliedsearchpartners.com> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] ASCP Certified Pathologist Assistant Job Opening- > Message-ID: <d3577a10.215b8%meli...@alliedsearchpartners.com> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I have a Full Time/Permanent job opening for an ASCP certified Pathologist > Assistant in Ohio. Please contact me for details. Have a great day! > > Melissa Owens > President, Laboratory Staffing > Allied Search Partners > > > T: 888.388.7571 ext. 102 > > F: 888.388.7572 > > > > ------------------------------ > > Message: 10 > Date: Tue, 10 May 2016 08:44:33 -0700 > From: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Melanin Bleach > Message-ID: > <d329219d3b967447a9e3ed3242117e3216826a1...@phx-msg-007-n1.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I need some help. Yesterday I bleached some heavily pigmented tissue. I > have to run some IHC's on them. I bought the bleaching kit from American > Master Tech. I had to put the Permanganate for about 6 hours to get the > melanin and then a couple of minutes in Oxalic Acid. I had to let them set > overnight because I could not get another IHC run in that day. It looks > like the tissue fell off during decloaking. I used Apex slides. I rarely > ever have to bleach anything here. So I am not sure if I did this correctly. > I am thinking I need to bleach and do the run in the same day and not let > them set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error. Any further review, > dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please notify > us immediately by reply email. Thank you." > > > > > > ------------------------------ > > Message: 11 > Date: Tue, 10 May 2016 16:33:26 +0000 > From: Jeffrey Robinson <jrobin...@pathology-associates.com> > To: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Melanin Bleach > Message-ID: > > <204A03EB5A7F0A4BB1EEDD52A963829C90A16313@PAEXCH1.PathologyAssociates.local> > > Content-Type: text/plain; charset="us-ascii" > > Hi Karen- I have also had problems getting tissue being stained for IHC > markers to adhere to the slide. The Potassium permanganate is really harse > on the tissue (if you can get the sections to stay on). Here is a trick I > picked up at an NSH lecture that I have used successfully several times. > > Run your IHC stains as usual. Rinse well in water. Do not counterstain with > hematoxylin. Stain with DiffQuik II for 30 seconds. Rinse in water for a > short period of time. Differentiate with 10 dips each in 2 changes of 95% > ETOH and 2 changes of 100% ETOH and then clear in xylene and coverslip. > Results: melanin pigment will turn green. Brown DAB stain will be > unaffected. Other tissue elements will be stained a medium blue color. > This method will also work for other Special Stains but I have not attempted > to modify this method for use on "Red" stains. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -----Original Message----- > From: Heckford, Karen - SMMC-SF via Histonet > [mailto:histonet@lists.utsouthwestern.edu] > Sent: Tuesday, May 10, 2016 8:45 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Melanin Bleach > > Good Morning, > I need some help. Yesterday I bleached some heavily pigmented tissue. I > have to run some IHC's on them. I bought the bleaching kit from American > Master Tech. I had to put the Permanganate for about 6 hours to get the > melanin and then a couple of minutes in Oxalic Acid. I had to let them set > overnight because I could not get another IHC run in that day. It looks > like the tissue fell off during decloaking. I used Apex slides. I rarely > ever have to bleach anything here. So I am not sure if I did this correctly. > I am thinking I need to bleach and do the run in the same day and not let > them set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error. Any further review, > dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please notify > us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This email and attachments may contain PHI that is privileged and > confidential and is not intended for any unauthorized person. If you, the > reader, are not the intended recipient you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. Do not read the email but instead reply to the sender and destroy > the message and any attachments. Thank you. > > > > ------------------------------ > > Message: 12 > Date: Tue, 10 May 2016 16:36:54 +0000 (UTC) > From: Rene J Buesa <rjbu...@yahoo.com> > To: "Heckford, Karen - SMMC-SF" <karen.heckf...@dignityhealth.org>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Melanin Bleach > Message-ID: > <930716214.1612034.1462898214077.javamail.ya...@mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > You are right. Bleaching is a "rough" procedure for the "survival" of > sections and if on top of that you left the section overnight in DiH2O that > is a recipe for disaster, as the one you experienced. Try to do the whole > procedure during the same day.Additionally it seems to me that 6h in > potassium permanganate is too much, you should check the condition of the > sections every hour trying to minimize KMnO4?action?the least time > possible.Ren? > > On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via > Histonet" <histonet@lists.utsouthwestern.edu> wrote: > > > Good Morning, > I need some help.? Yesterday I bleached some heavily pigmented tissue.? I > have to run some IHC's on them.? I bought the bleaching kit from American > Master Tech.? I had to put the Permanganate for about 6 hours to get the > melanin and then a couple of minutes in Oxalic Acid.? I had to let them set > overnight because I could not get another IHC run in that day.? It looks like > the tissue fell off during decloaking.? ? I used Apex slides.? I rarely ever > have to bleach anything here.? So I am not sure if I did this correctly.? I > am thinking I need to bleach and do the run in the same day and not let them > set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > ? ? Caution:? This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error.? Any further review, > dissemination, distribution, or copying of this message is strictly > prohibited.? If you have received this communication in error, please notify > us? immediately by reply email.? Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 150, Issue 13 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
