We are also interested in a reasonablly price and simple document control system. Thanks Gary
-----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, July 07, 2016 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Document control system: (J B) 2. Re: Samples not staining with Hematoxylin or primary (Teri Johnson) 3. Re: Media Lab (Lester Raff MD) 4. Re: Media Lab (J B) 5. MITF (Baldwin, Kathy) 6. Samples not staining with Hematoxylin or primary (Anna Huntley Coffey) 7. CWD testing (Johnson, Carole) 8. Why is Auromine heated in Histology, but not in Microbiology (Eddie Martin) 9. Re: MITF (Eddie Martin) 10. Special Stain (Diana Martinez-Longoria) ---------------------------------------------------------------------- Message: 1 Date: Wed, 06 Jul 2016 17:23:18 +0000 From: J B <craiga...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Document control system: Message-ID: <CANyXu7DTTkWNYNF8ABaAQG9q=3ybxgbopobep7zrus8ummq...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB ------------------------------ Message: 2 Date: Wed, 6 Jul 2016 17:55:30 +0000 From: Teri Johnson <tejohn...@genoptix.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: <6e205ab6e3544c63abb55c8fec2da...@phuscb-sp37mb01.genoptix.org> Content-Type: text/plain; charset=WINDOWS-1252 Anna wrote: I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"? Thanks group, Angie Hi Anna, If your dispensers are working properly, I would bet it's your slides. There are known issues with overly hydrophobic slides causing issues on the Ventana platform. They recommend you use Plus (+) slides manufactured from Erie Scientific. There may be others I am not aware of, but we use these slides for all IHC sectioned here and have had no issues on our Ventanas. Good luck! Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Wed, 6 Jul 2016 18:12:32 +0000 From: Lester Raff MD <lr...@uropartners.com> To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Media Lab Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E78B6A@COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" We have been using MediaLab for several years, beginning with their online courses and progressing to Document Control and InspectionProof. We are awaiting our first CAP inspection in which I expect the inspectors to be able to get virtually all the information they need from our MediaLab site. Also, customer support is great! It took a bit of time for the lab staff to accept the concept of online manuals and SOP's but in the long run it is a big win. -------------------------- Latest blog post: http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ -------------------------- Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: J B via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, July 06, 2016 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Document control system: Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 4 Date: Wed, 06 Jul 2016 18:47:56 +0000 From: J B <craiga...@gmail.com> To: Lester Raff MD <lr...@uropartners.com>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Media Lab Message-ID: <canyxu7c6fn0spwdoy1slxne9gaajajk12uu24bvak1rcbwu...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Thank you for the feedback. Was it hard or time consuming to upload all procedures? I will only have a few weeks before my CAP window starts. Thank you, JB On Wed, Jul 6, 2016, 11:26 AM Lester Raff MD via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > We have been using MediaLab for several years, beginning with their online > courses and progressing to Document Control and InspectionProof. We are > awaiting our first CAP inspection in which I expect the inspectors to be > able to get virtually all the information they need from our MediaLab site. > Also, customer support is great! > > > > It took a bit of time for the lab staff to accept the concept of online > manuals and SOP's but in the long run it is a big win. > > > > -------------------------- > > > > Latest blog post: > http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ > > > > -------------------------- > > Lester J. Raff, MD MBA > > UroPartners > > Medical Director Of Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel: 708-486-0076 > > Fax: 708-492-0203 > > > > -----Original Message----- > From: J B via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, July 06, 2016 12:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Document control system: > > > > Does anyone have experience with Media Labs document control system? I am > looking for good software for documentation control. Any feedback is > appreciated. > > > > Sincerely, > > > > JB > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Wed, 6 Jul 2016 20:12:30 +0000 From: "Baldwin, Kathy" <sbald...@mhhcc.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] MITF Message-ID: <1163fb0a386e4a22afebbd3d8d841...@exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" Hi Histonetters Was wondering if anyone has had any experience with MITF what controls would you use I have a fairly new pathologist that wants us to use Nevus' instead on melanoma and half the cases are staining and the other half are not what is your experience? Any help would be greatly appreciated! Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Wed, 6 Jul 2016 16:14:26 -0400 From: Anna Huntley Coffey <annamhunt...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: <cahncdcfehtkeympevg7yv8vvu7l8tm9gwsmpvd7esgnthzd...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Thanks so much to everyone who responded! A couple people have suggested that this issue may be due to a disruption to the charge on the slide (due to high temp baking, water bath additives, etc) which causes a hydrophobic barrier to form and prevents reagent from reaching the tissue. This sounds very convincing to me, but we have found that repeated antigen retrieval using a pressure cooker (our standard retrieval for this particular primary) has allowed 2 of the 3 slides to stain with both DAB and Hematoxylin. Would repeated retrieval be able to reverse the slide charge back to normal? I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with either GranzB or Hematoxylin. My controls and samples that did stain positive for GranzB look fine and the machine has been recently serviced and working well with other runs. I'd really appreciate your input if you have any advice or suggestions on what could have gone wrong. Thanks, Anna ------------------------------ Message: 7 Date: Wed, 6 Jul 2016 21:25:16 +0000 From: "Johnson, Carole" <cjohn...@nmda.nmsu.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] CWD testing Message-ID: <f00d04066b62f04480a441b7460c17b5ec113...@ex-mbx-p1.acn.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I would like to hear from people who are doing CWD testing on biopsies (tonsil or rectal). Are you placing a disclaimer on results, and if so do you include the number of follicles present in the specimen? You can answer privately at: cjohn...@nmda.nmsu.edu<mailto:cjohn...@nmda.nmsu.edu> Thanks for your input Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnositc Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ------------------------------ Message: 8 Date: Wed, 6 Jul 2016 23:45:01 -0500 From: Eddie Martin <edmarti...@gmail.com> To: "histonet (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Why is Auromine heated in Histology, but not in Microbiology Message-ID: <077d5dfb-890a-4acd-89f9-8ec66eb3b...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi Angie, The simplest answer, would be to save time. Whether heated, or microwaved at 80 power for 45 seconds, would be quicker than flooding the slide and letting stand for extended periods of time. ------------------------------ Message: 9 Date: Thu, 7 Jul 2016 00:46:25 -0500 From: Eddie Martin <edmarti...@gmail.com> To: "histonet (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] MITF Message-ID: <24f35fb0-a7e9-4261-bde0-27b4f1ad8...@gmail.com> Content-Type: text/plain; charset=us-ascii Hi Kathy, The Journal of Investigative Dermatology had an article in their December 2011 Volume 131 issue 12 on Human Cutaneous Melanomas lacking MITF and melanocyte differentiation. According to the article, Melanomas could be MITF and Melan-A negative, if the Melanomas were TAM tyrosine kinase receptor positive. In this particular study, 38% of the total Cutaneous Melanomas were Axl+ (member of TAM), and none of the Axl+ were MITF and Melan-A positive. That being said, it may explain why some of your cutaneous melanomas are not MITF positive. Known internal control tissue for MITF includes epithelioid tissue, mast cells, reactive histiocytes and osteoclasts stain intensely for MITF. A nevus would also be an ideal control tissue, as would any other perivascular epithelioid tumor. Best, Eddie Martin, HTL, HT(ASCP), QIHC ------------------------------ Message: 10 Date: Thu, 7 Jul 2016 12:49:29 +0000 From: Diana Martinez-Longoria <diana.martinez-longo...@ecrmc.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Special Stain Message-ID: <12b71261212be94bb9fb7735484b9fa178c8b...@exmbx01.ecrmc.ci.el-centro.ca.us> Content-Type: text/plain; charset="us-ascii" Good morning everyone, I was wondering if anyone can help us in regards to special stain contamination. We will like to know if contamination can happen as a result of reagents touching the writing of a marking pen on a slide? For example we are manually running a slide that has the typical marking pen writing on the slide for identification and we are doing a silver stain, will it contaminate and not give an accurate result? I know this might be a weird question, but any information, resources, documentation you can direct me to would be very much appreciated. Please email me privately at dmlongo...@ecrmc.org<mailto:dmlongo...@ecrmc.org>. Thank you! ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 5 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
