Ann, I would recommend validating manual microtomes more often then automatic. Any traditional gear driven microtome does change overtime as gears wear. This eventually will lead to the micron indicator on the microtome not reading what you are truly cutting. Automated, microtomes driven by stepper motors tend to remain accurate longer but you may want to document the accuracy every couple of years.
To check calibration most service engineers will mount a micrometer to the microtome and measure the feed of the microtome as the hand wheel turns. The procedure generally takes 5 minutes. Check with your microtome manufacture to see if they can assist you. Robert Jacox Thermo Fisher Scientific Commercial Marketing Manager Sent from my iPad > On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Has anyone had to validate a microtome (manual/automatic). If so, how did > you do it? > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
