Lisa,
I have used all of the previously mentioned methods for cleaning molds. It
really depends on the facilities that you have access to. Boiling in hot soapy
water is great for deep cleaning, but you may not have enough pots or beakers
to clean them all at once. Xylene, followed by 100% is good, but you may not
have it in the budget to use fresh xylene and forget to save some used.
Sometimes having a container big enough to do this is also a challenge.
Spraying them down with mold release after air drying, but then you have to
remember to allow them to dry. Placing them in the processor can clog the
lines, but if you place them in the oven first to drain and only do a few at a
time that can help.
We have our students boil them and then dry them in the oven. Weekend dry in
the oven, and on Monday am they shake them up and put them away.
Some techs don't like the residue of mold release. It doesn't bother me
though.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481
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Message: 1
Date: Thu, 14 Jul 2016 10:18:12 +0000
From: "Kennedy, Lisa" <lisakenn...@catholichealth.net>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Cleaning Tissue Molds
Message-ID:
<517c2a781e654047a5ec98d17390f9f25d939...@chiex003.chi.catholichealth.net>
Content-Type: text/plain; charset="us-ascii"
Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds? We do
not want to use our processor due to its age and wear and tear and frequent
replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)
This email and attachments contain information that may be confidential or
privileged. If you are not the intended recipient, notify the sender at once
and delete this message completely from your information system. Further use,
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privilege or other confidentiality protections.
------------------------------
Message: 2
Date: Thu, 14 Jul 2016 07:56:43 -0400
From: "Hannen, Valerie" <valerie.han...@parrishmed.com>
To: "'Kennedy, Lisa'" <lisakenn...@catholichealth.net>
Cc: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E60B@isexstore03>
Content-Type: text/plain; charset="us-ascii"
We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30
minutes( with occasional stirring to clear the xylene), then rinse in H2O, let
dry(laid out) and spray with the mold release.
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com
Message: 3
Date: Thu, 14 Jul 2016 13:35:22 +0000
From: <idimi...@mun.ca>
To: <valerie.han...@parrishmed.com>, <lisakenn...@catholichealth.net>
Cc: <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID:
<14c3108e8b98ef43b3edae58336700340202495...@exchange.med.mun.ca>
Content-Type: text/plain; charset="us-ascii"
We used to do that until 5 years ago, when we started looking in cleaning the
molds without the use of Xylene. We found that we don't need to clan them, we
turn them down on a metal tray covered with paper towel and put them in our 60
degree C oven overnight. That gets rid of the wax left on the molds. Then the
next morning we spray them with mold release and they are ready for use.
Now, bear in mind we do only research, so I don't know if you can do this in a
hospital, there may be possible contamination or other issues, but it perfect
solution for us.
I. Dimitrova, MLT, LHP, B.Tech., M.Sc.
Histology Supervisor
Faculty of Medicine
Memorial University of Newfoundland
St. John's, NL Canada
-----Original Message-----
From: Hannen, Valerie via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: July-14-16 9:27 AM
To: 'Kennedy, Lisa'
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cleaning Tissue Molds
We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30
minutes( with occasional stirring to clear the xylene), then rinse in H2O, let
dry(laid out) and spray with the mold release.
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com
-----Original Message-----
From: Kennedy, Lisa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 14, 2016 6:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cleaning Tissue Molds
Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds? We do
not want to use our processor due to its age and wear and tear and frequent
replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)
Message: 4
Date: Thu, 14 Jul 2016 14:11:18 +0000 (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: "Kennedy, Lisa" <lisakenn...@catholichealth.net>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cleaning Tissue Molds
Message-ID:
<1673797881.3661474.1468505478591.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Place your molds in a 2% dishwasher soap boiling solution for 5 minutes ? was
in running water for 5 minutes ? dry in a convection oven at 60?C for 10
minutes and your molds will be ready to use.As a "release" solution use a
mixture 1:1 of 2-propanol and mineral oil (light weight).Ren?
On Thursday, July 14, 2016 6:34 AM, "Kennedy, Lisa via Histonet"
<histonet@lists.utsouthwestern.edu> wrote:
Dear Fellow Histo Techs,
What is the BEST practice for cleaning the paraffin block tissue molds?? We do
not want to use our processor due to its age and wear and tear and frequent
replacement of cellanoids when we clean them via the processor.
Thanks so much for your help! In advance.
Sincerely,
Lisa Kennedy, HT (ASCP)
This email and attachments contain information that may be confidential or
privileged. If you are not the intended recipient, notify the sender at once
and delete this message completely from your information system. Further use,
disclosure, or copying of information contained in this email is not
authorized, and any such action should not be construed as a waiver of
privilege or other confidentiality protections.
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