Dears, I was wondering if some of you might have experience in the following:
We have had a lot of problems cutting WAT tissue either directly embedded in OCT or pre-fixed with PFA or formalin (either cold or and them embedded with OCT. We solved this problem following Ryan Berry et al, 2014 paper protocol with gelatin. We get really nice cuts with that, but when performing the staining with oil red, we can not remove the gelatin from the cut and the oil red stays on the top of the tissue cut, it looks like there an oil-aqueous phase the does not let the oil red enter into the tissue.... Any suggest for removing the gelatin and for letting the oil red entering into the tissue will be more than welcome! Many thanks and kind regards, Mònica -- Mònica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguil...@irbbarcelona.org <neus.pr...@irbbarcelona.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet