Angela, I replace saffron with Gardenia Fruits Extract (Tokyo Chemical Industry, TCI America in US, C1527) for Movat's and HES. It has no C.I. number, but contains crocin dye (CAS 42553-65-1) extract the dye with ethanol (5-6g/100ml) for 2-3days and filtrate. I hope this will help for you
Masahiro HIRATA Department of Diagnostic Pathology, Kyoto University Hospital Kyoto, JAPAN hirat...@kuhp.kyoto-u.ac.jp 2016-09-02 2:00 GMT+09:00 <histonet-requ...@lists.utsouthwestern.edu>: > Message: 7 > Date: Wed, 31 Aug 2016 18:24:15 -0700 > From: Angela Lamberth <alambe...@lji.org> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Modified Movat's Pentachrome stain > Message-ID: > <CANUNjWkzBGuwsm-Vh07X69b=kbustekqz9+ajhub5ja0m20...@mail.gm > ail.com> > Content-Type: text/plain; charset=UTF-8 > > Hi Histonetters! > > I?m gearing up to perform a pentachrome stain. I will be making this in > house and not using a kit. Through searching histonet, I?ve found a > protocol used by the Children?s Hospital of Philadelphia Pathology Core. > http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf > > > Two things: Iodine crystals are out of the question. Can I replace that > with Lugol?s iodine solution or should I just substitute Weigert?s for the > hematoxylin in this protocol? > > > Also, saffron vs tartrazine vs Orange G stain. There is a real difference > in price. Does anybody have a personal preference in terms of quality or > aesthetics? Is paying the extra money for saffron really worth it? > > > Thanks! > > Angela > ============================= 平田勝啓 (Hirata Masahiro) 京都大学医学部附属病院病理診断科 075-751-3491 hirat...@kuhp.kyoto-u.ac.jp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet