RBS is the same as Methylene Blue, I believe.
_____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Alicia Marie Ortega [mailto:alicia.ort...@colorado.edu] Sent: Thursday, September 22, 2016 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Sanderson Rapid Bone Stain Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
