Hello Histo-netters! I have a Bond RX and before I try to recreate the wheel, I thought I would ask some experts. Has anyone used a goat primary on this instrument and if so how? Also, has anyone had success with TUNEL on this instrument and if so, could you share your knowledge? Thank you all for your time, Cathy Cathy M. Mathis Laboratory Technician IV
Department of Pathology - Section on Comparative Medicine Clarkson Campus Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.1538 cmmat...@wakehealth.edu -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, September 28, 2016 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 154, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Water for H&E Stainers? (Hannen, Valerie) 2. Position announcement (Leslie, Mary) 3. Sandersons Rapid Bone stain chemistry and history (Gayle Callis) 4. Re: water for stainers (Terri Braud) 5. H.pylori question (Gareth Davis) 6. buffered zn-formalin recipe needed (Preiszner, Johanna) 7. Re: GMS (Caroline Miller) 8. Re: H.pylori question (Kienitz, Kari) 9. ATRX Antibody (Marcum, Pamela A) 10. Re: Water for H&E Stainers? (cfitz) 11. Re: Water for H&E Stainers? (WILLIAM DESALVO) 12. Re: Histonet Digest, Vol 154, Issue 22 (Nasir Abbas) 13. HT grossing competencies (Farah Nawaz) 14. looking for a cryostat service tech. in the Houston area. (Hebert, Pamela) 15. EXAKT band saw blade (Garcia, Lori, M.Sc.) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Sep 2016 13:10:05 -0400 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> To: 'P Sicurello' <pat...@gmail.com> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E63B@isexstore03> Content-Type: text/plain; charset="us-ascii" Ours is hooked up to DI water. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -----Original Message----- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 12:28 PM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Message: 2 Date: Tue, 27 Sep 2016 17:13:54 +0000 From: "Leslie, Mary" <mary.les...@tricore.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Position announcement Message-ID: <e0d95e77cca6d74c9618b5ddc3458c98e4867...@trl-mail-ww.tricore.org> Content-Type: text/plain; charset="us-ascii" Histotechnologist needed M-F 1900 -0330 Tricore Reference Laboratories Albuquerque, NM Contact: laura.enriq...@tricore.org<mailto:laura.enriq...@tricore.org> 505-938-8915 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 11:19:17 -0600 From: "Gayle Callis" <gayle.cal...@bresnan.net> To: "Histonet" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Sandersons Rapid Bone stain chemistry and history Message-ID: <004a01d218e3$47811390$d6833ab0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" You worte: RBS is the same as Methylene Blue, I believe. Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: -----Original Message----- From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ] Sent: Thursday, September 22, 2016 4:50 PM To: histonet at lists.utsouthwestern.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Subject: [Histonet] Question on Sanderson Rapid Bone Stain Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder *********************************************** Sandersons Rapid Bone Stain is oxidized methylene blue. It is actually a modified Stevenels blue, a polychromatic stain made by oxidizing methylene blue with potassium permanganate that produces the byproducts i.e. methylene violet, azure A, azure B, thionin, toluidine blue, thionin and possibly some other thiazine dyes .Stevenels blue was first used by Maniatopoulos et al to stain PMMA embedded, un-etched bone sections using a 60?C water bath and optional counterstaining with either Van Gieson or basic fuchsin. Cathy Sanderson (Mayton) found a better, easier way to make up Stevenels blue for large production. We found it tends to be a weaker stain unless you etch the bone with 0.5% formic acid using a sonicator for 1 minute, rinse with hot tap water very briefly, blot and look at the surface stained bone. Over aggressive water rinsing allows the stain to release from this mildly acid etched calcified bone. All the etching is doing is a gentle removal of calcium from only a few micrometers of bone surface and allow the stain to penetrate better. Acid etching intensified RBS staining but there are drawbacks but ways to deal with that. You can stain etched bone sections, view, photograph and then do a very brief (only a few quick dips), blot after counterstain, view and photograph the now counterstained section. Avoiding over rinsing keeps the RBS in etched bone section. If things don?t look right, merely polish the stain from surface and start over. You can counterstain Sanderson RBS stained (etched) bone section with the two mentioned counterstains, but it has to be done very briefly or the counterstain will differentiate RBS from the bone and be overly red. RBS, as used according to Sanderson and product brochure instructions, is for un-etched mineralize bone sections in PMMA or even EXAKT preparations. We preferred to acid etch, do the original Stevenels Blue instead of RBS, and get a much deeper stain on osteoid, and other bone components. Counterstaining was not done as it masked acid etched bone components excessively or removed them, The joy of using RBS is avoiding a long, tedious, and messy making up of Stevenels blue. One can also try to stain longer in RBS per brochure instructions to see if that deepens the staining. Old bone picks up the stain differently than young bone in terms of age of the animal. Different species can stain differently too. It is important to know that repeated heating of RBS (or Stevenels) allows the portassium permanganate to continue oxidizing the methylene blue, and also keeps raising the pH to be more alkaline. One should replenish or top off the stain, always filter it into a clean coplin jar before using or reusing this stain. Eventually the stain does not work very well and you need to start with new stock. We preferred to use a MacNeals tetrachrome combined with toluidine blue per Sterchi method for more brilliant staining of osteoid other bone components including cartilage. Take care Gayle Callis HTL/HT/MT(ASCP) ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 17:36:25 +0000 From: "Terri Braud" <tbr...@holyredeemer.com> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] water for stainers Message-ID: <48E053DDF6CE074DB6A7414BA05403F808DA66@HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Our Prisma is hooked up to tap water. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Water for H&E Stainers? (P Sicurello) 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello <pat...@gmail.com> Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 ------------------------------ Message: 5 Date: Tue, 27 Sep 2016 10:45:25 -0700 From: Gareth Davis <garethdavisy...@gmail.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H.pylori question Message-ID: <CAHx9TZPjeVZH_Pyrw2Hi9faRWJQ12OG8TD5zhB_ULhebj6=z...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ------------------------------ Message: 6 Date: Tue, 27 Sep 2016 18:21:12 +0000 From: "Preiszner, Johanna" <preis...@mail.etsu.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] buffered zn-formalin recipe needed Message-ID: <mwhpr07mb3134bf1f305a0c29cfd682639c...@mwhpr07mb3134.namprd07.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Hi, For the life of me I can't locate a recipe for buffered zn-formalin, nor online or off. Could someone please help us out? Thanks, Hanna Preiszner ETSU/QCOM ------------------------------ Message: 7 Date: Tue, 27 Sep 2016 11:23:47 -0700 From: Caroline Miller <mi...@3scan.com> To: "Martin, Gary" <gmar...@marshallmedical.org> Cc: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] GMS Message-ID: <cafthrqp3meq-2yo6cxkhewxew5vekcik7gq0-jtv21rzte1...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Maybe a fixation issue? Is this happening on all your slides, or just one set. Also, do you have a little acetic acid in the light green, I find that helps. I also find it washes out quickly and a fast dehydration is necessary. Very funny that you are having issues with the LG and not the silver, lol, histology can be so 'interesting' sometimes! mills On Mon, Sep 26, 2016 at 4:30 PM, Martin, Gary via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I am having difficulties with a stain I've done many times ...GMS. The > light green is only staining the outer edges of the specimen. Any Ideas? > > > Thanks > > Gary > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 8 Date: Tue, 27 Sep 2016 19:06:22 +0000 From: "Kienitz, Kari" <kkien...@orclinic.com> To: Gareth Davis <garethdavisy...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] H.pylori question Message-ID: <cy1pr10mb0716d6ee18bfeedc68df863aaf...@cy1pr10mb0716.namprd10.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Local Coverage Determinations (LCD) from the website for the Centers for Medicare & Medicaid Services (CMS). Please use the link below to access this policy. you may find this information useful. Look at the GI section. It specifically calls out the use of special stains for H.pylori. Reimbursement may be an issue for you. L35693 MolDX: Special Histochemical Stains and Immunohistochemical Stains Palmetto GBA(11302) http://www.cms.gov/medicare-coverage-database/details/lcd-details.aspx?LCDId=35693&ver=6&ContrId=229&ContrVer=1 N Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Gareth Davis via Histonet [histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H.pylori question Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 27 Sep 2016 19:55:03 +0000 From: "Marcum, Pamela A" <pamar...@uams.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] ATRX Antibody Message-ID: <8856c26e8b844c50adb842ca38fd7...@mail13m2n1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" I am asking this question for our IHC Supervisor. She is looking for a source for the ATRX antibody as either an RUO or preferably IVD. They use the Ventana Ultras and Benchmarks so if anyone has a procedure that would be great. Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 10 Date: Tue, 27 Sep 2016 14:17:08 -0700 From: cfitz <cf...@007group.com> To: Elizabeth Chlipala <l...@premierlab.com>, P Sicurello <pat...@gmail.com>, "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: <yn8vcl1ski3k31ssywl67qd0.1475011028...@email.android.com> Content-Type: text/plain; charset=utf-8 Our Prisma is connected to tap water with a prefilter that we change weekly.? CathyBritish Columbia? Sent from my Samsung Galaxy smartphone. -------- Original message --------From: Elizabeth Chlipala via Histonet <histonet@lists.utsouthwestern.edu> Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello <pat...@gmail.com>, "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 27 Sep 2016 16:01:08 -0700 From: WILLIAM DESALVO <wdesalvo....@outlook.com> To: cfitz <cf...@007group.com>, Liz Chlipala <l...@premierlab.com>, P Sicurello <pat...@gmail.com>, histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: <col129-w432debebcb390844f785c482...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Tap water is most often used in H&E staining for rinsing and sometimes blueing and can be very cost effective. I do suggest you use a filter prior to the water entering the instrument. The filter can eleviate one of the key concerns, contaminates. The typical contaminates/compounds can be: Inorganic ions: chlorides, nitrates, sulfates, sodium, calcium, iron Organic molecules: humic acids, phenols, tannins, pesticide residues Particles and colloids Microorganisms and their by-products Dissolved gases The other key concern is that the pH of the tap water may vary by location and even season. To change the color of hematoxylin stained nuclei from redish to blue, the pH should be 7 or higher. Most tap water is typically slightly acidic (pH of around 6.0 to 6.8), but this is much more alkaline than the pH of the haematoxylin, around pH 2.7. Rinsing for two to five minutes in running tap water will remove most of the excess mordant giving sharp blue nuclear staining. If you want sharper and better defined As my good friend Skip Brown states, a good H&E stain is "balance of coloration". Understand the chemistry and you control the balance. William DeSalvo, BS HTL(ASCP) > Date: Tue, 27 Sep 2016 14:17:08 -0700 > To: l...@premierlab.com; pat...@gmail.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Water for H&E Stainers? > From: histonet@lists.utsouthwestern.edu > > Our Prisma is connected to tap water with a prefilter that we change weekly. > CathyBritish Columbia > > > Sent from my Samsung Galaxy smartphone. > -------- Original message --------From: Elizabeth Chlipala via Histonet > <histonet@lists.utsouthwestern.edu> Date: 2016-09-27 9:54 AM (GMT-08:00) > To: P Sicurello <pat...@gmail.com>, "'histonet@lists.utsouthwestern.edu' > (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] Water for H&E Stainers? > Paula > > We have ours hooked up to tap water. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Tuesday, September 27, 2016 10:28 AM > To: HistoNet > Subject: [Histonet] Water for H&E Stainers? > > Good Morning Everyone, > > > > What type of water do you use with your automated H&E stainers? House or > deionized/distilled? > > > > We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. > L Thank you in advance. > > Sincerely, > > > > Paula > > > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, retransmission, > dissemination or other use of or taking of any action in reliance upon this > information by persons or entities other than the intended recipient is > prohibited. If you received this e-mail in error, please contact the sender > and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 28 Sep 2016 08:39:40 +0000 (UTC) From: Nasir Abbas <shinwar...@yahoo.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Histonet Digest, Vol 154, Issue 22 Message-ID: <1743394579.715511.1475051980...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response.?Thanks in advance. Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 On Wednesday, 28 September 2016, 1:12, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. GMS (Martin, Gary) ? 2. Re: Unprocessing tissue (Caroline Miller) ? 3. Water for H&E Stainers? (P Sicurello) ? 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Sep 2016 16:30:49 -0700 From: "Martin, Gary" <gmar...@marshallmedical.org> To: <histonet@lists.utsouthwestern.edu> Subject: [Histonet] GMS Message-ID: ??? <6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local> Content-Type: text/plain;??? charset="us-ascii" I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary ------------------------------ Message: 2 Date: Mon, 26 Sep 2016 16:49:14 -0700 From: Caroline Miller <mi...@3scan.com> To: "Armstrong, Karoleigh T" <karoleigh.armstr...@armc.net> Cc: "histonet@lists.utsouthwestern.edu" ??? <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Unprocessing tissue Message-ID: ??? <CAFTHRQMn5=hqzoyf-frj-mf2agzeup_gzwyjxnhgux90hmk...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Caroline, > > This may sound a little crazy, > > but I was working on some mummified tissue back in the 80's, (Mommy is > old), and to deal with the dried out tissue we would give them, > > 1. A quick .5 percent Sodium Hydroxide soak, 5minutes > > 2. Wash is running water, 1 minute > > 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes > > 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. > > 5. Wash with running water 2-3 minutes. > > Proscess your tissue as you usually would. > > They came out pretty darn good. > > KK Armstrong H.T. (ASCP) > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello <pat...@gmail.com> To: HistoNet <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Water for H&E Stainers? Message-ID: ??? <capsjddyceta1fldsufgwzctayssqk6rxdrvtssqjmdwqaju...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 10:54:18 -0600 From: Elizabeth Chlipala <l...@premierlab.com> To: P Sicurello <pat...@gmail.com>, ??? "'histonet@lists.utsouthwestern.edu' ??? (histonet@lists.utsouthwestern.edu)" ??? <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: ??? <14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 22 ***************************************** ------------------------------ Message: 13 Date: Wed, 28 Sep 2016 14:02:38 +0000 From: Farah Nawaz <fna...@uhcc.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] HT grossing competencies Message-ID: <bn6pr18mb147582550ad8166c8ac54f87ad...@bn6pr18mb1475.namprd18.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Hello: I am a new AP supervisor and I am looking to add grossing duties to our HTs. They are currently being trained here in our lab. Can someone please share an example of non-pathologist grossing annual competency checklist with me? I just need a reference to create our own competency checklist for our HTs that gross. Thank you, Farah Nawaz, CT(ASCP), MA-Mgmt Anatomic Pathology Supervisor Union Hospital of Cecil County Office: 410-392-7084 Fax: 443-674-1596 fna...@uhcc.com CONFIDENTIALITY STATEMENT. This email and any attachment is for the sole use of the intended recipient and may contain private, confidential and/or privileged information that may be subject to Union Hospital of Cecil County internal policies. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you have received this transmission in error, please notify Union Hospital of Cecil County immediately by return email or by email to administra...@uhcc.com and delete the message and all copies, printouts and attachments from your system. ------------------------------ Message: 14 Date: Wed, 28 Sep 2016 14:40:01 +0000 From: "Hebert, Pamela" <pheb...@shrinenet.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] looking for a cryostat service tech. in the Houston area. Message-ID: <c4adea684afb4eca8ab82b00b078d...@idc-ex-11.shriners.cc> Content-Type: text/plain; charset="us-ascii" I am looking for a cryostat service tech in the Houston/Galveston area to do a PM. Any recommendations would be appreciated. Pam Hebert, HT(ASCP) Shriners Hospitals for Children- Galveston, TX ------------------------------ Message: 15 Date: Wed, 28 Sep 2016 16:32:56 +0000 From: "Garcia, Lori, M.Sc." <lori.gar...@medtronic.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] EXAKT band saw blade Message-ID: <b7663f03f32a45d4808d0f44ea0e2...@mspm1bmsgm11a.ent.core.medtronic.com> Content-Type: text/plain; charset="us-ascii" Looking for an EXAKT band saw blade for a colleague due to a backorder. If anyone has one to loan out or sell, please contact me. Thanks, Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 23 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
