As Greg has already mentioned, placing control sections on each patient slide will assist in troubleshooting your staining issue. Joanne, I don't believe you mentioned it in your initial email, but did you attempt a repeat and if so, did the results replicate on the repeat? Occasionally there may be a mixing issue or other physical issue with the covertile that inhibits complete reaction across the slide.
Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada ________________________________________ From: Joanne Clark via Histonet [histonet@lists.utsouthwestern.edu] Sent: Tuesday, November 29, 2016 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
