Hi Jamie,
1. The sheets have adhesive part and holding non-adhesive part. The sheet is as big as a regular A4 size. The length of the individual tapes is fixed but you can cut the sheets in different widths depending on the width of the sample. You can also order sheets with different fixed lengths. Probably 20-30 sectioning tapes can be generated from one sheet. 2. Nobody knows the chemistry of the transfer film except the creator. 3. You can use any media if you dehydrate you can use permanent or if not aqueous media. Their media is better optimized for the tape optics for imaging. 4. Regular OCT does not produce as good sections. SCEM works great. SCEM L1 is new and improved product. I have not tried. 5. The 2C cryofilms are the old versions. The new and improved version is 3C(16UF). The improved version produces better sections. Mesruh Turkekul > I am looking into trying the Kawamoto's film method. ?I have the order > sheet for the cryofilm sheets and other supplies. ?I did have a few > questions. > 1. ?I was wondering if anyone knows when you order a sheet how many slides > can be produced from that sheet? ?2. ?Does anyone know what the transfer > film is??3. ?What are the advantages to using their SCCM mounting medium > ?and the difference between the four types offered G1, R1, R2, R3?4. ?What > are the advantages of using heir SCEM embedding medium and the difference > between the SCEM and SCEM L1? ?5. ?Does anyone know the differences of the > cryofilm 2C(9), 2C(10), and 3C(16UF)? > Thanks so much for any information you may have. > Jamie?? > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet