http://www.agilent.com/cs/library/technicaloverviews/public/08002_ihc_staining_methods.pdf
-----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, February 09, 2017 10:51 AM To: Jennifer <histot...@dermspec.com>; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] hand staining immunos You do not have to "babysit" the procedure for it has well defined/timed steps. You just need a timer and check the slides when required. As to procedural "dos & donts" try to get a copy of the DAKO IHC manual.René On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <histonet@lists.utsouthwestern.edu> wrote: Hello, My dermatopathologist wants me to start hand staining immunos for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech to babysit the stain and also about the consistency of the quality in the end result. Your opinions are much appreciated. Thank you! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
