Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together
:) mills On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi, > the blades are packed with a tiny, bit of oil. This can cause you to not > obtain a ribbon. > Try gently cleaning with xylene, followed by alcohol before > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX > 405-759-7513www.excaliburpathology.com > > From: Gareth Davis via Histonet <histonet@lists.utsouthwestern.edu> > To: "Histonet@lists.utsouthwestern.edu" <histonet@lists. > utsouthwestern.edu> > Sent: Tuesday, April 11, 2017 11:55 AM > Subject: [Histonet] No Ribbon > > I feel like I should be able to figure this one out, but I have been having > trouble with getting a ribbon for a while now. I have a Leica Microtome > RM2125 and I use either a AccuEdge blade (I get one block to cut then it > starts to compress) or a StatCut blade (sections do not stick together at > all), both High Profile. The angle is about 4 microns. I change the angle > a lot to see if that makes a difference and it doesn't. There are so many > factors that could be the issue, I think lack of humidity may be the main > one. So, suggestions please!! > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet