Hi Greg, I use BONDS and Apex slides as well. What we do with tissue that
washes (usually heavy tumor and some harsher stains like CD34) is leave the
tissue unbaked at room temp for 12 hours and then bake at 60 degrees for 30
minutes. I don't know if you are including your control tissue on the same
slide (we do) but we also cut a separate slide with just patient sections and
that seems to help with extra paraffin runoff from the control tissue
underneath the patient tissue. We've been doing this successfully for years.
I hope this is helpful, I totally understand your frustration!
Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
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From: histonet-requ...@lists.utsouthwestern.edu
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Sent: Thursday, July 13, 2017 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 164, Issue 9
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Today's Topics:
1. Re: Section adherence issues (IHC) (Walter Benton)
2. Permanent/Full Time Histotech Job in Panama City, FL
(Melissa Owens)
3. New Lab Related Blog Post (Lester Raff MD)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Jul 2017 17:37:38 +0000
From: Walter Benton <wben...@cua.md>
To: "Hujet, Matthew"
<https://urldefense.proofpoint.com/v2/url?u=http-3A__Matthew.Hujet-40ssmhealth.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=F1TSqbvH_gNcZCNxbzfj4YvTHIhCktr_3GoGHgornJM&e=
>, Greg Dobbin
<https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e=
>
Cc: "kgevele...@ihis.org" <kgevele...@ihis.org>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Section adherence issues (IHC)
Message-ID: <816e042155824c4193f79c50c1461...@mail01.gcu-MD.local>
Content-Type: text/plain; charset="us-ascii"
Thanks. This is helpful information.
-----Original Message-----
From: Hujet, Matthew [mailto:matthew.hu...@ssmhealth.com]
Sent: Wednesday, July 12, 2017 1:35 PM
To: Walter Benton <wben...@cua.md>; Greg Dobbin
<https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e=
>
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Section adherence issues (IHC)
We have had really good luck with both the TOMO and Millennia 2000 adhesion
slides. We initially tried using the TOMO slides for everything (IHC, special
stains, and H&E's), but found that there was too much background staining on
the specials and H&E's. For IHC, the TOMO slides were great. We then switched
to the Millennia 2000 for everything, and they have been working really well
across the board. With regards to just IHC, I personally feel that the TOMO
slides are better, but if you only want one type of glass in your lab, the
Millennia 2000 are the way to go.
Matthew Hujet
Histology Technical Specialist
SSM Health St. Mary's Hospital - Madison
700 S. Park St.
Madison, WI 53715
Please note my new email address: matthew.hu...@ssmhealth.com
-----Original Message-----
From: Walter Benton [mailto:wben...@cua.md]
Sent: Wednesday, July 12, 2017 10:34 AM
To: Greg Dobbin
<https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e=
>
Cc: kgevele...@ihis.org; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section adherence issues (IHC)
Greg,
We are running into a similar issue on the Biocare Nemesis platform and the
support there suggest humidity to be the issue. Our sections stay adhered to
the slides through depar, antigen retrieval, but appear to fall off during the
staining process. It is not consistent. We are going to try some other slides
and see if the issue persists. I'd be interested to hear your findings and
resolution.
Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
-----Original Message-----
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)
Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here
for each other when needed!
My problem is section adherence during IHC staining. And it is not all of the
time it is intermittent; so not all of the time and not on all slides when it
does occur.
Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for
our IHC stains. We have no additives in our water baths. Our sections drain in
a stand and then any trapped water under the section is either flicked or
wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining. We do not do on board baking
(primarily because on board baking is only 10 mins long and with the section
lifting issue we want longer).
Some of the specimen types are more susceptible it would seem. Cervix LEEP
specimens tend to be bad, we use a 4mm punch to obtain some of our control
tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad
one and sometimes our ER/PR control sections (but not always). Fine needle
cores where a lot of tumour is present tend to be bad (again not always).
We have tried baking longer, we turn ourselves inside out trying to get all of
the water out from under the sections, we have tried charged slides from
another manufacturer. I have looked at the HIER protocols and none are
extraordinary in nature. We use very clean covertiles (no scratches or
blemishes). Our specimens are fixed for 24hrs before processing. We use the
same slides for Special Stains and don't have this issue there.
I need some new suggestions to try! All ideas welcome.
Cheers,
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A 1P0
*Everything in moderation...even moderation itself**!*
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------------------------------
Message: 2
Date: Wed, 12 Jul 2017 19:59:14 +0000
From: Melissa Owens <meli...@alliedsearchpartners.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Permanent/Full Time Histotech Job in Panama City,
FL
Message-ID: <d58bf7c8.413f4%meli...@alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"
Hello All,
I have a full time/permanent position for a Histotech in Panama City, FL. This
is a Monday-Friday Day Shift opportunity and I am looking for a long term
permanent candidate. Please let me know if you are interested and I will
provide more information. Thank you!
Melissa Owens
President, Laboratory Staffing
Allied Search Partners
Direct Line: 407.413.9117
Toll Free: 888.388.7571 ext. 102
F: 888.388.7572
------------------------------
Message: 3
Date: Thu, 13 Jul 2017 11:43:46 +0000
From: Lester Raff MD <lr...@uropartners.com>
To: "Histonet (histonet@lists.utsouthwestern.edu)"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] New Lab Related Blog Post
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF114F70C9@COLOEXCH01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
Good morning to all. The rains in Chicago have finally stopped. Link for a lab
blog post is below.
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chicagonow.com_downsize-2Dmaybe_2017_07_oops-2Dwe-2Ddid-2Dit-2Dagain-2Danother-2Dresearch-2Dpaper-2Dtells-2Dus-2Dpathologists-2Dsometimes-2Dhave-2Dit-2Dtough-2Dthis-2Dtime-2Dwith-2Dmelanoma_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=BMnYbAIiXA3i7op2GB24dtCro4-e1ZM2MHR_w-FW3gc&e=
Thanks,
Lester J. Raff, MD MBA
Laboratory Medical Director
------------------------------
Subject: Digest Footer
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