Exactly...and then it wont shrivel up.... C
On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic <dunat...@sbcglobal.net> wrote: > I agree with Colleen, processing is the issue. You need to process on a > longer program in order to dehydrate and clear the agar properly. > dusko > > > On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Be sure you are processing them on an overnight process. Even if the > samples are small it takes that time to process the agar properly. > > I use Histogel and learned this lesson the hard way. My samples always > "shriveled" up too until someone enlightened me about the processing time. > I would think agar might be the same. > > > Just a thought, > > Colleen Forster HT(ASCP)QIHC > U of MN > > On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > I would try histogel instead of agar. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > l...@premierlab.com<mailto:l...@premierlab.com> > > www.premierlab.com<http://www.premierlab.com/> > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g> > > Longmont, CO 80504 > <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g> > > > > From: Heather Marlatt via Histonet [mailto:histonet@lists. > > utsouthwestern.edu] > > Sent: Tuesday, December 19, 2017 9:12 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Mouse bladder > > > > Does anyone have a good protocol for mouse bladder embedded in agar that > > they are willing to share? We have mouse bladders in 2% agar and have > tried > > a few different protocol variations from our usual mouse but the agar > keeps > > shriveling up. > > > > Thanks > > Heather > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists. > utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet